研究者情報

トウゴウ　タツル   TOGO TATSURU   東郷　建    所属   医学部医学科 解剖学（生物学）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells
掲載誌名	正式名：Biology Open 略　 称：Biol Open ISSNコード：20466390
掲載区分	国外
巻・号・頁	6(12),1814-1819頁
著者・共著者	Togo Tatsuru
担当区分	筆頭著者,責任著者
発行年月	2017/12
概要	Disruption of cellular plasma membranes is a common event in many animal tissues, and the membranes are usually rapidly resealed. Moreover, repeated membrane disruptions within a single cell reseal faster than the initial wound in a protein kinase A (PKA)- and protein kinase C (PKC)-dependent manner. In addition to wounded cells, recent studies have demonstrated that wounding of Madin-Darby canine kidney (MDCK) cells potentiates membrane resealing in neighboring cells in the short-term by purinergic signaling, and in the long-term by nitric oxide/protein kinase G signaling. In the present study, real-time imaging showed that cell membrane disruption stimulated cAMP synthesis and Ca2+ mobilization from intracellular stores by purinergic signaling in neighboring MDCK cells. Furthermore, inhibition of PKA and PKC suppressed the ATP mediated short-term potentiation of membrane resealing in neighboring cells. These results suggest that cell membrane disruption stimulates PKA and PKC via purinergic signaling to potentiate cell membrane resealing in neighboring MDCK cells.
DOI	10.1242/bio.028977
PMID	29092813